RLU stands for Relative Light Unit and is the unit of measure used in bioluminescence. Luminometers measure and quantify light with an RLU output. Because manufacturers use different sensor technologies and algorithms for adding up the photons, RLU measurements will vary from system to system. However, because the ATP bioluminescence reaction is linear, the more ATP present means the more light will be present. (Comparing RLU values is like comparing Fahrenheit and Celsius; they are two different scales for the same temperature.) Using Hygiena’s SystemSURE Plus and UltraSnap ATP swabs: 1 RLU = 1 femtomole ATP (1x10 -15 mole)
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